Iq¬ Irjn

Iq¬ IĨÀ sN¿pó hn[w

Snjyp IĨÀ coXnbnemWv Iq¬ IĨÀ Dïm¡póXv. AWphnapàamb kmlNcy§fpÅ (sterile condition) Hcp IĨÀapdn kÖam¡n AhnsS h¨mWv IĨÀ sNt¿ïXv. CXn\mbn Ct¸mgpÅ Hcp apdnbpsS tImWnð Xsó ss¹hpUv sImtïm atäm 1.5 x 1.5 x 2.25 aoäÀ Afhnð Hcp sNdnb apdn sI«nbSbv¡pIbmWv \ñXv. CXn\v Hcp hmXnð am{Xw hbv¡pI. aäp shântejs\mópw AcpXv. Cu apdnbv¡pÅnð ssa¡ H«n¨tXm Kvfmkv joäv ]Xn¨tXm Bb Hcp sNdnb tai Dïmbncn¡Ww. Hcp UV em¼pw km[mcW Hcp Syq_v sseäpw apdn¡I¯v kÖam¡Ww. apdnbpsS shfnbnð \nópw UV em¼v Hm¬ sN¿m³ ]äpó hn[w kzn¨v {IaoIcn¡pI. CXmWv Hcp IĨÀ apdnbpsS amXrI.

IĨÀ sNt¿ï Iq¬ Hgn¨v _m¡n BhiyapÅ Hcp _p¬k¬ _ÀWÀ, sterile PDA slants, inoculation needle, 0.1% saÀ¡pdn¡v t¢mssdUv emb\n sterile petridish ð FSp¯Xv, cïv Unjnð sterile water Fónhsbñmw IĨÀ apdn¡I¯v tai¸pd¯psh¨v IXIS¨tijw UV em¼v CSWw. ]nóoSv Ac aWn¡qÀ Ignªv UV em¼v AW¨n«v IĨÀ sN¿m\pÅ Iq¬, 90% ip²amb Bð¡tlmÄ sImïv ]pdw XpS¨p hr¯nbm¡nb tijw IĨÀapdnbnse tai¸pd¯v hbv¡Ww. ]nsó sÌdnssekv sNbvX Hcp »bnUv sImïv Iq¬ s\SpsI cïmbn ]nfÀóv IqWnsâ Xïpw IpS`mKhpw tNcpó Øe¯v DÅnð \nópw Hcp sNdnb IjWw apdns¨Sp¡pI. CXns\ hoïpw sNdnb IjW§fm¡n apdn¨v Hmtcmópw 0.1% saÀ¡pdnIv t¢mssdUv emb\nbnð 30 sk¡âv hoXw ap¡nh¨n«v DS³Xsó sÌssdð shůnð cïp {]mhiyw \ómbn IgpInsbSp¡Ww. C§s\ surface sterilize sNbvX Iq¬ IjW§Ä ap³Iq«n sÌdnssekv sNbvX sSÌvSyq_pIfnð h¨n«pÅ PDA bnte¡v sÌdnssekv sNbvX Hcp innoculation needle sImïv FSp¯pshbv¡pI. sSÌv Syq_nse cotton plug Xpd¡póXv _p¬k¬ _ÀWÀ ^vsfbnan\v apIfnð h¨mbncn¡Ww. Iq¬ IjWw Syq_n\It¯bv¡v needle sImïv h¨mepS³Xsó cotton plug sImïv ASbv¡pIbpw thWw. 

C§s\ PDA aoUnb¯nð h¨ Iq¬ IjW§Ä aqóp Znhkw Ignbpt¼mÄ shfp¯ ]q¸embn hfcm¬ XpS§pw.  HmcmgvNsImïv Syq_n\I¯pÅ aoUnbw \ndsb shfp¯ ]q¸ð (mycelium) sImïv \ndªncn¡pw. CXmWv IqWnsâ IĨÀ (pure culture). shÅ \nd¯neñmsX, ]¨tbm, Idpt¸m, ]nt¦m, aªtbm \ndw hcpóXmbmð IĨÀ Zqjys¸«p (contaminated) t]mbXmbn IcpXmw. IĨÀ apdnbnð Ibdpt¼mÄ ]mZc£Ifqcn hbv¡pIbpw, ssI \ómbn sUtämtfm, Bð¡tlmtfm sImïp IgpIn hr¯nbm¡pIbpw sN¿Ww.